ecl solution (Bio-Rad)
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ecl solution
Ecl Solution, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 20862 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/clarity+western+ecl+substrate/Clarity+ECL+Western+Substrate/pm42898112-257-241-243
Average 99 stars, based on 20862 article reviews
Ecl Solution, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 20862 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/clarity+western+ecl+substrate/Clarity+ECL+Western+Substrate/pm42898112-257-241-243
Average 99 stars, based on 20862 article reviews
ecl solution - by Bioz Stars,
2026-10
99/100 stars
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Western Blot:Article Title: Multimodal substrate recruitment enables CTLH MKLN1 E3 ligase to target acetylated N-, C-, and internal degrons Article Snippet: The following secondary HRP-conjugated antibodies were used: Goat anti-Mouse IgG (H + L) Secondary Antibody (Thermo Fisher Scientific # 31430), Goat anti-Rabbit IgG (H + L) Secondary Antibody (Thermo Fisher Scientific # 31460). .. Immunoblots were developed using Article Title: Alternative splicing fine-tunes modular protein interfaces to control protein localization. Article Snippet: .. The membranes were then developed with Article Title: Bacillus-based probiotics ameliorate bleomycin-induced pulmonary fibrosis in rats: coupling intestinal barrier reinforcement to the modulation of M2 macrophage polarization/TGF-β/p38MAPK and inflammasome-driven pyroptosis. Article Snippet: Pulmonary fibrosis (PF) is a chronic, progressive lung disorder with an 87.5% mortality rate recorded in aged patients.. Emerging evidence bridges gut dysbiosis to PF pathogenesis.. The consumption of a Bacillus-based probiotic exhibited immunomodulatory and anti-inflammatory effects by alleviating gut dysbiosis and restoring gut microbiome composition, positioning it as a promising therapeutic strategy for gut-organ axis disorders. Article Title: IL-1β/IL-6 inflammatory crosstalk drives DNA methylation and histone remodeling associated with cytokine induced senescence in luminal breast cancer Article Snippet: .. The blots were visualized using Article Title: The isolated HEPN domain of SACSIN exhibits RNA-binding activity. Article Snippet: The membrane was blocked with 5% milk dissolved in 0.05% TWEEN-TBS 1 × and then incubated over night at 4 °C with one of the following primary antibodies: anti-FLAG M2 (F1804, Merck Life Science); Anti-SACSIN 1 3 antibody (EPR11864, Abcam); anti-GAPDH (14C10, Cell Signaling); anti-Vinculin (A2752, ABclonal). .. The following day, after three washing steps of 5 min with 0.05% TWEEN-TBS 1x, the membrane was incubated for 1 h with the HRP-conjugated secondary antibody (1706515 and 1706516, BIO-RAD Laboratories), washed for additional three washing steps of 5 min with 0.05% TWEEN-TBS 1 × and then revealed by chemiluminescence using the Article Title: Heterologous Expression and Immunoreactivity of Wheat Amylase-Trypsin Inhibitors. Article Snippet: The purified proteins were concentrated using Amicon Ultra-15 spin columns with a cut-off of 10 kDa (Merck), while at the same time, the buffer was exchanged for 0.05 M PBS (pH 7.8) containing 0.2 M NaCl to remove imidazole. .. Although the presence and purity of the His6-tagged ATI proteins were confirmed by SDS-PAGE (Supplementary Figure S2B) and Western blot using 6× His tag antibodies (Invitrogen), anti-rabbit secondary antibodies (Active Motif, Inc., Carlsbad, CA, USA) and Article Title: A Peculiar Phospholipid-Binding Specificity and Cell Death-Related Properties of Tubic ( Nicotiana tabacum Tubby-like F-Box Protein 8). Article Snippet: Detection of the EGFP- and mRFP-containing proteins was performed using monoclonal anti-EGFP 3A9 antibody [56] and rabbit polyclonal anti-tRFP antibodies (Evrogen, Moscow, Russia; at 1:2000 dilution), respectively, in combination with secondary anti-mouse or anti-rabbit IgG linked to HRP (Thermo Scientific, Waltham, MA, USA). .. Chemiluminescence detection was performed with Article Title: The effect of lignocellulosic biomass pretreatment by-products on the fermentation activity of Saccharomyces cerevisiae yeast, measured using indices of toxic stress. Article Snippet: Then, after washing the membranes with TBST (1xTBS, 0.5 ml L− 1 Tween20), they were incubated with the secondary antibody (Goat Anti-Mouse IgG (H + L)- HRP Conjugate Cat# 170-6516 or Goat Anti-Rabbit IgG (H + L)- HRP ConjugateCat# 170- 6515, Bio-Rad, Hercules, California, USA). .. For digital imaging of the obtained results, Imaging:Article Title: IL-1β/IL-6 inflammatory crosstalk drives DNA methylation and histone remodeling associated with cytokine induced senescence in luminal breast cancer Article Snippet: .. The blots were visualized using Article Title: The isolated HEPN domain of SACSIN exhibits RNA-binding activity. Article Snippet: The membrane was blocked with 5% milk dissolved in 0.05% TWEEN-TBS 1 × and then incubated over night at 4 °C with one of the following primary antibodies: anti-FLAG M2 (F1804, Merck Life Science); Anti-SACSIN 1 3 antibody (EPR11864, Abcam); anti-GAPDH (14C10, Cell Signaling); anti-Vinculin (A2752, ABclonal). .. The following day, after three washing steps of 5 min with 0.05% TWEEN-TBS 1x, the membrane was incubated for 1 h with the HRP-conjugated secondary antibody (1706515 and 1706516, BIO-RAD Laboratories), washed for additional three washing steps of 5 min with 0.05% TWEEN-TBS 1 × and then revealed by chemiluminescence using the Article Title: A Peculiar Phospholipid-Binding Specificity and Cell Death-Related Properties of Tubic ( Nicotiana tabacum Tubby-like F-Box Protein 8). Article Snippet: Detection of the EGFP- and mRFP-containing proteins was performed using monoclonal anti-EGFP 3A9 antibody [56] and rabbit polyclonal anti-tRFP antibodies (Evrogen, Moscow, Russia; at 1:2000 dilution), respectively, in combination with secondary anti-mouse or anti-rabbit IgG linked to HRP (Thermo Scientific, Waltham, MA, USA). .. Chemiluminescence detection was performed with Article Title: The effect of lignocellulosic biomass pretreatment by-products on the fermentation activity of Saccharomyces cerevisiae yeast, measured using indices of toxic stress. Article Snippet: Then, after washing the membranes with TBST (1xTBS, 0.5 ml L− 1 Tween20), they were incubated with the secondary antibody (Goat Anti-Mouse IgG (H + L)- HRP Conjugate Cat# 170-6516 or Goat Anti-Rabbit IgG (H + L)- HRP ConjugateCat# 170- 6515, Bio-Rad, Hercules, California, USA). .. For digital imaging of the obtained results, Membrane:Article Title: The isolated HEPN domain of SACSIN exhibits RNA-binding activity. Article Snippet: The membrane was blocked with 5% milk dissolved in 0.05% TWEEN-TBS 1 × and then incubated over night at 4 °C with one of the following primary antibodies: anti-FLAG M2 (F1804, Merck Life Science); Anti-SACSIN 1 3 antibody (EPR11864, Abcam); anti-GAPDH (14C10, Cell Signaling); anti-Vinculin (A2752, ABclonal). .. The following day, after three washing steps of 5 min with 0.05% TWEEN-TBS 1x, the membrane was incubated for 1 h with the HRP-conjugated secondary antibody (1706515 and 1706516, BIO-RAD Laboratories), washed for additional three washing steps of 5 min with 0.05% TWEEN-TBS 1 × and then revealed by chemiluminescence using the Incubation:Article Title: The isolated HEPN domain of SACSIN exhibits RNA-binding activity. Article Snippet: The membrane was blocked with 5% milk dissolved in 0.05% TWEEN-TBS 1 × and then incubated over night at 4 °C with one of the following primary antibodies: anti-FLAG M2 (F1804, Merck Life Science); Anti-SACSIN 1 3 antibody (EPR11864, Abcam); anti-GAPDH (14C10, Cell Signaling); anti-Vinculin (A2752, ABclonal). .. The following day, after three washing steps of 5 min with 0.05% TWEEN-TBS 1x, the membrane was incubated for 1 h with the HRP-conjugated secondary antibody (1706515 and 1706516, BIO-RAD Laboratories), washed for additional three washing steps of 5 min with 0.05% TWEEN-TBS 1 × and then revealed by chemiluminescence using the SDS Page:Article Title: Heterologous Expression and Immunoreactivity of Wheat Amylase-Trypsin Inhibitors. Article Snippet: The purified proteins were concentrated using Amicon Ultra-15 spin columns with a cut-off of 10 kDa (Merck), while at the same time, the buffer was exchanged for 0.05 M PBS (pH 7.8) containing 0.2 M NaCl to remove imidazole. .. Although the presence and purity of the His6-tagged ATI proteins were confirmed by SDS-PAGE (Supplementary Figure S2B) and Western blot using 6× His tag antibodies (Invitrogen), anti-rabbit secondary antibodies (Active Motif, Inc., Carlsbad, CA, USA) and Purification:Article Title: Heterologous Expression and Immunoreactivity of Wheat Amylase-Trypsin Inhibitors. Article Snippet: The purified proteins were concentrated using Amicon Ultra-15 spin columns with a cut-off of 10 kDa (Merck), while at the same time, the buffer was exchanged for 0.05 M PBS (pH 7.8) containing 0.2 M NaCl to remove imidazole. .. Although the presence and purity of the His6-tagged ATI proteins were confirmed by SDS-PAGE (Supplementary Figure S2B) and Western blot using 6× His tag antibodies (Invitrogen), anti-rabbit secondary antibodies (Active Motif, Inc., Carlsbad, CA, USA) and Labeling:Article Title: The effect of lignocellulosic biomass pretreatment by-products on the fermentation activity of Saccharomyces cerevisiae yeast, measured using indices of toxic stress. Article Snippet: Then, after washing the membranes with TBST (1xTBS, 0.5 ml L− 1 Tween20), they were incubated with the secondary antibody (Goat Anti-Mouse IgG (H + L)- HRP Conjugate Cat# 170-6516 or Goat Anti-Rabbit IgG (H + L)- HRP ConjugateCat# 170- 6515, Bio-Rad, Hercules, California, USA). .. For digital imaging of the obtained results, |
