clarity tm western ecl substrate (Bio-Rad)
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clarity tm western ecl substrate
Clarity Tm Western Ecl Substrate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 2720 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/clarity+western+ecl+substrate/Clarity+Max+Western+ECL+Substrate/pmc13169231-128-6-11
Average 97 stars, based on 2720 article reviews
Clarity Tm Western Ecl Substrate, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 2720 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/clarity+western+ecl+substrate/Clarity+Max+Western+ECL+Substrate/pmc13169231-128-6-11
Average 97 stars, based on 2720 article reviews
clarity tm western ecl substrate - by Bioz Stars,
2026-09
97/100 stars
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Incubation:Article Title: Empagliflozin modulates microRNA expression in human primary cardiomyocytes under CoCl 2 -induced hypoxia Article Snippet: .. After subsequent incubation with secondary antibodies (dilution 1:10,000) (Cat. No. A9044 anti-Mouse IgG for GAPDH; Cat. No. sc-2357 anti-Rabbit IgG for HIF1α), immunopositive signals were visualized using Article Title: Chicken interleukin-26: characterization and antigen capture enzyme-linked immunosorbent assay Article Snippet: The membranes were blocked with Superblock Blocking Buffer (Thermo Scientific, Waltham, MA) and incubated with 1 μg/ml of anti-chIL-26 mAbs (#5D11, #6C3, #7H10, #8A5, and #10C7). .. After incubation with horseradish peroxidase (HRP)-conjugated rabbit anti-mouse IgG secondary antibody (Jackson ImmunoResearch Laboratories Inc., USA) diluted 1:1000 in blocking buffer, the membranes were visualized using Article Title: Vaccination modality and injection site influence the immune response and protection against sea lice Lepeophtheirus salmonis infestation in Atlantic salmon ( Salmo salar ) Article Snippet: .. Western Blot:Article Title: Empagliflozin modulates microRNA expression in human primary cardiomyocytes under CoCl 2 -induced hypoxia Article Snippet: .. After subsequent incubation with secondary antibodies (dilution 1:10,000) (Cat. No. A9044 anti-Mouse IgG for GAPDH; Cat. No. sc-2357 anti-Rabbit IgG for HIF1α), immunopositive signals were visualized using Article Title: Ketone body metabolism activates the immune response against Staphylococcus aureus infection by fueling the tricarboxylic acid cycle and affecting histone β-hydroxybutyrylation Article Snippet: .. Protein bands were visualized using the Article Title: Secreted enzyme uptake masks the in vivo phenotype of macrophage-specific lysosomal acid lipase deletion Article Snippet: .. Secondary polyclonal goat anti-rabbit antibody (P0448, 1:1,000; Agilent Technologies, Inc., Santa Clara, CA) conjugated to HRP was visualized using the Article Title: Targeted mutagenesis of the ICP4 transactivation domain generates a protective DIVA vaccine against duck plague Article Snippet: Alexa Fluor 488-conjugated goat anti-rabbit IgG (H + L) secondary antibody (Invitrogen, A11008) and Alexa Fluor 568-conjugated goat anti-mouse IgG (H + L) secondary antibody (Invitrogen, A11004). .. The other reagents used and their sources were as follows: liposome transfection reagent (YESEN, 40802ES03), TransDetect Double-Luciferase Reporter Assay Kit (Transgen, TR202-02), RNA-easy TM Isolation Reagent (Vazyme, R701-01), SuperMix for qPCR (gDNA digester plus) (YEASEN, 11141ES60), qPCR SYBR Green Master Mix (YEASEN, 11184ES08), protein A/G Magnetic Beads (MedChemExpress, HY-K02),Sonication ChIP Kit (Abclonal, RK20258), DNAiso reagent (Takara, Cat No. 9770A), HiPure Viral DNA Kit (Magen, Cat No. 3191), ClonExpress II One Step Cloning Kit (Vazyme, Cat No. C112-01), Article Title: Genetic inactivation of TRPM4 does not alter the temperature-dependent movement of mouse microglia Article Snippet: After three washes with phosphate buffered saline with Tween 20 (PBS-T), the membrane was incubated for 1 h at room temperature with an anti-rabbit IgG horseradish peroxidase (HRP)-linked antibody (1:1000, Cell Signaling Technology). .. After incubating the membrane for 5 min with Article Title: Hypermethylation-induced silencing of ITGA4 promotes oral squamous cell carcinoma progression through SNX5 upregulation Article Snippet: The membranes were washed three times with TBST (Tris-buffered saline with 0.1% Tween 20) and incubated with an anti-rabbit IgG conjugated with horseradish peroxidase (W4018) or an anti-mouse IgG conjugated with horseradish peroxidase (cat. no. W4028; 1:2,500 dilution) from Promega Corporation for 1 h at room temperature. .. Protein signals were visualized using Article Title: Chicken interleukin-26: characterization and antigen capture enzyme-linked immunosorbent assay Article Snippet: The membranes were blocked with Superblock Blocking Buffer (Thermo Scientific, Waltham, MA) and incubated with 1 μg/ml of anti-chIL-26 mAbs (#5D11, #6C3, #7H10, #8A5, and #10C7). .. After incubation with horseradish peroxidase (HRP)-conjugated rabbit anti-mouse IgG secondary antibody (Jackson ImmunoResearch Laboratories Inc., USA) diluted 1:1000 in blocking buffer, the membranes were visualized using Article Title: Vaccination modality and injection site influence the immune response and protection against sea lice Lepeophtheirus salmonis infestation in Atlantic salmon ( Salmo salar ) Article Snippet: .. Imaging:Article Title: Empagliflozin modulates microRNA expression in human primary cardiomyocytes under CoCl 2 -induced hypoxia Article Snippet: .. After subsequent incubation with secondary antibodies (dilution 1:10,000) (Cat. No. A9044 anti-Mouse IgG for GAPDH; Cat. No. sc-2357 anti-Rabbit IgG for HIF1α), immunopositive signals were visualized using Article Title: Ketone body metabolism activates the immune response against Staphylococcus aureus infection by fueling the tricarboxylic acid cycle and affecting histone β-hydroxybutyrylation Article Snippet: .. Protein bands were visualized using the Article Title: Secreted enzyme uptake masks the in vivo phenotype of macrophage-specific lysosomal acid lipase deletion Article Snippet: .. Secondary polyclonal goat anti-rabbit antibody (P0448, 1:1,000; Agilent Technologies, Inc., Santa Clara, CA) conjugated to HRP was visualized using the Article Title: Genetic inactivation of TRPM4 does not alter the temperature-dependent movement of mouse microglia Article Snippet: After three washes with phosphate buffered saline with Tween 20 (PBS-T), the membrane was incubated for 1 h at room temperature with an anti-rabbit IgG horseradish peroxidase (HRP)-linked antibody (1:1000, Cell Signaling Technology). .. After incubating the membrane for 5 min with Article Title: Hypermethylation-induced silencing of ITGA4 promotes oral squamous cell carcinoma progression through SNX5 upregulation Article Snippet: The membranes were washed three times with TBST (Tris-buffered saline with 0.1% Tween 20) and incubated with an anti-rabbit IgG conjugated with horseradish peroxidase (W4018) or an anti-mouse IgG conjugated with horseradish peroxidase (cat. no. W4028; 1:2,500 dilution) from Promega Corporation for 1 h at room temperature. .. Protein signals were visualized using Article Title: Chicken interleukin-26: characterization and antigen capture enzyme-linked immunosorbent assay Article Snippet: The membranes were blocked with Superblock Blocking Buffer (Thermo Scientific, Waltham, MA) and incubated with 1 μg/ml of anti-chIL-26 mAbs (#5D11, #6C3, #7H10, #8A5, and #10C7). .. After incubation with horseradish peroxidase (HRP)-conjugated rabbit anti-mouse IgG secondary antibody (Jackson ImmunoResearch Laboratories Inc., USA) diluted 1:1000 in blocking buffer, the membranes were visualized using Article Title: Vaccination modality and injection site influence the immune response and protection against sea lice Lepeophtheirus salmonis infestation in Atlantic salmon ( Salmo salar ) Article Snippet: .. Transfection:Article Title: Targeted mutagenesis of the ICP4 transactivation domain generates a protective DIVA vaccine against duck plague Article Snippet: Alexa Fluor 488-conjugated goat anti-rabbit IgG (H + L) secondary antibody (Invitrogen, A11008) and Alexa Fluor 568-conjugated goat anti-mouse IgG (H + L) secondary antibody (Invitrogen, A11004). .. The other reagents used and their sources were as follows: liposome transfection reagent (YESEN, 40802ES03), TransDetect Double-Luciferase Reporter Assay Kit (Transgen, TR202-02), RNA-easy TM Isolation Reagent (Vazyme, R701-01), SuperMix for qPCR (gDNA digester plus) (YEASEN, 11141ES60), qPCR SYBR Green Master Mix (YEASEN, 11184ES08), protein A/G Magnetic Beads (MedChemExpress, HY-K02),Sonication ChIP Kit (Abclonal, RK20258), DNAiso reagent (Takara, Cat No. 9770A), HiPure Viral DNA Kit (Magen, Cat No. 3191), ClonExpress II One Step Cloning Kit (Vazyme, Cat No. C112-01), Reporter Assay:Article Title: Targeted mutagenesis of the ICP4 transactivation domain generates a protective DIVA vaccine against duck plague Article Snippet: Alexa Fluor 488-conjugated goat anti-rabbit IgG (H + L) secondary antibody (Invitrogen, A11008) and Alexa Fluor 568-conjugated goat anti-mouse IgG (H + L) secondary antibody (Invitrogen, A11004). .. The other reagents used and their sources were as follows: liposome transfection reagent (YESEN, 40802ES03), TransDetect Double-Luciferase Reporter Assay Kit (Transgen, TR202-02), RNA-easy TM Isolation Reagent (Vazyme, R701-01), SuperMix for qPCR (gDNA digester plus) (YEASEN, 11141ES60), qPCR SYBR Green Master Mix (YEASEN, 11184ES08), protein A/G Magnetic Beads (MedChemExpress, HY-K02),Sonication ChIP Kit (Abclonal, RK20258), DNAiso reagent (Takara, Cat No. 9770A), HiPure Viral DNA Kit (Magen, Cat No. 3191), ClonExpress II One Step Cloning Kit (Vazyme, Cat No. C112-01), Isolation:Article Title: Targeted mutagenesis of the ICP4 transactivation domain generates a protective DIVA vaccine against duck plague Article Snippet: Alexa Fluor 488-conjugated goat anti-rabbit IgG (H + L) secondary antibody (Invitrogen, A11008) and Alexa Fluor 568-conjugated goat anti-mouse IgG (H + L) secondary antibody (Invitrogen, A11004). .. The other reagents used and their sources were as follows: liposome transfection reagent (YESEN, 40802ES03), TransDetect Double-Luciferase Reporter Assay Kit (Transgen, TR202-02), RNA-easy TM Isolation Reagent (Vazyme, R701-01), SuperMix for qPCR (gDNA digester plus) (YEASEN, 11141ES60), qPCR SYBR Green Master Mix (YEASEN, 11184ES08), protein A/G Magnetic Beads (MedChemExpress, HY-K02),Sonication ChIP Kit (Abclonal, RK20258), DNAiso reagent (Takara, Cat No. 9770A), HiPure Viral DNA Kit (Magen, Cat No. 3191), ClonExpress II One Step Cloning Kit (Vazyme, Cat No. C112-01), Real-time Polymerase Chain Reaction:Article Title: Targeted mutagenesis of the ICP4 transactivation domain generates a protective DIVA vaccine against duck plague Article Snippet: Alexa Fluor 488-conjugated goat anti-rabbit IgG (H + L) secondary antibody (Invitrogen, A11008) and Alexa Fluor 568-conjugated goat anti-mouse IgG (H + L) secondary antibody (Invitrogen, A11004). .. The other reagents used and their sources were as follows: liposome transfection reagent (YESEN, 40802ES03), TransDetect Double-Luciferase Reporter Assay Kit (Transgen, TR202-02), RNA-easy TM Isolation Reagent (Vazyme, R701-01), SuperMix for qPCR (gDNA digester plus) (YEASEN, 11141ES60), qPCR SYBR Green Master Mix (YEASEN, 11184ES08), protein A/G Magnetic Beads (MedChemExpress, HY-K02),Sonication ChIP Kit (Abclonal, RK20258), DNAiso reagent (Takara, Cat No. 9770A), HiPure Viral DNA Kit (Magen, Cat No. 3191), ClonExpress II One Step Cloning Kit (Vazyme, Cat No. C112-01), SYBR Green Assay:Article Title: Targeted mutagenesis of the ICP4 transactivation domain generates a protective DIVA vaccine against duck plague Article Snippet: Alexa Fluor 488-conjugated goat anti-rabbit IgG (H + L) secondary antibody (Invitrogen, A11008) and Alexa Fluor 568-conjugated goat anti-mouse IgG (H + L) secondary antibody (Invitrogen, A11004). .. The other reagents used and their sources were as follows: liposome transfection reagent (YESEN, 40802ES03), TransDetect Double-Luciferase Reporter Assay Kit (Transgen, TR202-02), RNA-easy TM Isolation Reagent (Vazyme, R701-01), SuperMix for qPCR (gDNA digester plus) (YEASEN, 11141ES60), qPCR SYBR Green Master Mix (YEASEN, 11184ES08), protein A/G Magnetic Beads (MedChemExpress, HY-K02),Sonication ChIP Kit (Abclonal, RK20258), DNAiso reagent (Takara, Cat No. 9770A), HiPure Viral DNA Kit (Magen, Cat No. 3191), ClonExpress II One Step Cloning Kit (Vazyme, Cat No. C112-01), Magnetic Beads:Article Title: Targeted mutagenesis of the ICP4 transactivation domain generates a protective DIVA vaccine against duck plague Article Snippet: Alexa Fluor 488-conjugated goat anti-rabbit IgG (H + L) secondary antibody (Invitrogen, A11008) and Alexa Fluor 568-conjugated goat anti-mouse IgG (H + L) secondary antibody (Invitrogen, A11004). .. The other reagents used and their sources were as follows: liposome transfection reagent (YESEN, 40802ES03), TransDetect Double-Luciferase Reporter Assay Kit (Transgen, TR202-02), RNA-easy TM Isolation Reagent (Vazyme, R701-01), SuperMix for qPCR (gDNA digester plus) (YEASEN, 11141ES60), qPCR SYBR Green Master Mix (YEASEN, 11184ES08), protein A/G Magnetic Beads (MedChemExpress, HY-K02),Sonication ChIP Kit (Abclonal, RK20258), DNAiso reagent (Takara, Cat No. 9770A), HiPure Viral DNA Kit (Magen, Cat No. 3191), ClonExpress II One Step Cloning Kit (Vazyme, Cat No. C112-01), Chromatin Immunoprecipitation:Article Title: Targeted mutagenesis of the ICP4 transactivation domain generates a protective DIVA vaccine against duck plague Article Snippet: Alexa Fluor 488-conjugated goat anti-rabbit IgG (H + L) secondary antibody (Invitrogen, A11008) and Alexa Fluor 568-conjugated goat anti-mouse IgG (H + L) secondary antibody (Invitrogen, A11004). .. The other reagents used and their sources were as follows: liposome transfection reagent (YESEN, 40802ES03), TransDetect Double-Luciferase Reporter Assay Kit (Transgen, TR202-02), RNA-easy TM Isolation Reagent (Vazyme, R701-01), SuperMix for qPCR (gDNA digester plus) (YEASEN, 11141ES60), qPCR SYBR Green Master Mix (YEASEN, 11184ES08), protein A/G Magnetic Beads (MedChemExpress, HY-K02),Sonication ChIP Kit (Abclonal, RK20258), DNAiso reagent (Takara, Cat No. 9770A), HiPure Viral DNA Kit (Magen, Cat No. 3191), ClonExpress II One Step Cloning Kit (Vazyme, Cat No. C112-01), Cloning:Article Title: Targeted mutagenesis of the ICP4 transactivation domain generates a protective DIVA vaccine against duck plague Article Snippet: Alexa Fluor 488-conjugated goat anti-rabbit IgG (H + L) secondary antibody (Invitrogen, A11008) and Alexa Fluor 568-conjugated goat anti-mouse IgG (H + L) secondary antibody (Invitrogen, A11004). .. The other reagents used and their sources were as follows: liposome transfection reagent (YESEN, 40802ES03), TransDetect Double-Luciferase Reporter Assay Kit (Transgen, TR202-02), RNA-easy TM Isolation Reagent (Vazyme, R701-01), SuperMix for qPCR (gDNA digester plus) (YEASEN, 11141ES60), qPCR SYBR Green Master Mix (YEASEN, 11184ES08), protein A/G Magnetic Beads (MedChemExpress, HY-K02),Sonication ChIP Kit (Abclonal, RK20258), DNAiso reagent (Takara, Cat No. 9770A), HiPure Viral DNA Kit (Magen, Cat No. 3191), ClonExpress II One Step Cloning Kit (Vazyme, Cat No. C112-01), Membrane:Article Title: Genetic inactivation of TRPM4 does not alter the temperature-dependent movement of mouse microglia Article Snippet: After three washes with phosphate buffered saline with Tween 20 (PBS-T), the membrane was incubated for 1 h at room temperature with an anti-rabbit IgG horseradish peroxidase (HRP)-linked antibody (1:1000, Cell Signaling Technology). .. After incubating the membrane for 5 min with Blocking Assay:Article Title: Chicken interleukin-26: characterization and antigen capture enzyme-linked immunosorbent assay Article Snippet: The membranes were blocked with Superblock Blocking Buffer (Thermo Scientific, Waltham, MA) and incubated with 1 μg/ml of anti-chIL-26 mAbs (#5D11, #6C3, #7H10, #8A5, and #10C7). .. After incubation with horseradish peroxidase (HRP)-conjugated rabbit anti-mouse IgG secondary antibody (Jackson ImmunoResearch Laboratories Inc., USA) diluted 1:1000 in blocking buffer, the membranes were visualized using |